Bile acids are essential for regulating cholesterol metabolism and the intestinal digestion and absorption of fat. Their signaling functions play an important role in chronic disorders, sepsis, or drug metabolism.
Biocrates Life Sciences has now developed an assay for the standardized analysis of 16 human specific and 19 mouse specific bile acids from sample sizes as small as 10 µL designed for research use.
The Biocrates Bile Acids Kit is based on (ultra) high performance liquid chromatography coupled with electrospray tandem mass spectrometry (UHPLC-MS/MS) and is characterized by quick and highly effective sample extraction on a 96-well patented filter plate.
To ensure accuracy and precision, the kit comes with a set of calibration standards, isotope-labeled internal standards, and quality control samples. The assay has been rigorously validated to European Medicines Agency (EMA) guidelines.