The company has selected Ulverston in Cumbria for the construction of the new biopharmaceutical factory.
The £500m investment will also include two manufacturing sites in Scotland at Montrose and Irvine.
The company is making other investments totalling £80m at its sites in Ware in Hertfordshire to increase manufacturing capacity for its respiratory inhalation device and at Barnard Castle in County Durham to establish a dermatology manufacturing centre of excellence.
GSK CEO Andrew Witty said the company’s first new UK factory will create up to 1,000 new jobs over the lifetime of the projects.
"We are also actively considering other investments in our UK manufacturing network which would create further jobs and reinforce the UK’s international competitiveness and as a world leader in life sciences," Witty added.