This study aimed to evaluate the effect of insulin on HEK293SF-3F6 cell growth in two types of media while increasing influenza production with the addition of different concentrations of insulin and quantifying influenza using a robust method.

HEK293SF-3F6 is a suspension-adapted cell line that has successfully been used for the production of the influenza virus, virus-like particles, lentivirus vectors, adenovirus and adeno-associated virus.

The cell line can be cultured in an in-house media and in commercially-available serum-free media but weak growth profiles are obtained in chemically defined media.

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