HPLC analysis of fluorescently labeled oligosaccharides is a widely used tool for biopharmaceutical glycoprofiling. In this process, glycans are released from the therapeutic glycoprotein then tagged with a fluorescent dye such as 2-AB (2-aminobenzamide) or 2-AA (2-aminobenzoic acid) by reductive amination prior to chromatography. Excess labeling reagents such as dye and reducing agent interfere with the HPLC analysis – so need to removed without disturbing the relative proportions of the various glycan species.
One of the most reliable methods for achieving post-labeling cleanup is use of LudgerClean S-cartridges. These employ a hydrophilic membrane to separate the labeled glycans from non-glycan contaminants and are routinely used in the biopharma industry during glycoprofiling QC for lot release of FDA and EMA approved biopharmaceuticals. They are also the method of choice in our laboratories for detailed characterisation of drug glycosylation patterns for regulatory submissions. S-cartridges work superbly well but they are slow and not suited for high throughput (HT) work.
Ludger has developed a fast, high-throughput system for post-labeling cleanup of glycan samples. The LudgerClean T1 cartridge system is designed for drug glycoprofiling analysts who want the reliability of S-cartridges but also want to process larger numbers of samples easily and get the results more quickly. The cartridges work with a Ludger-Velocity™ SPE vacuum manifold.
For more information please visit www.ludger.com/t1 and to request a quote or place an order, please contact info(at)ludger.com