The reason is that NeuAc is the desired, normal human-type sialylation, while NeuGc is found in non-human glycoproteins and is considered an undesired, aberrant form of sialylation for therapeutic glycoproteins. A widely used method for determining the NeuAc:NeuGc ratio is HPLC analysis of sialic acids labelled with fluorescent DMB (1,2-diamino-4,5-methylenoxybenzene).

Ludger is very pleased to announce the launch of two new quantitative standards, NeuAc (Cat# CM-NEU-AC-01) and NeuGc (Cat# CM-NEU-GC-01). These are quantitative standards handled using NIST-F calibrated apparatus and calibrated against USP traceable Neu5Ac and Neu5Gc monosaccharides respectively using DMB analysis kits (Cat# LT-KDMB-A1) and a LudgerSep UHPLC column (Cat# LS-UR2-2.1×100). Serial dilutions of these standards can be used to obtain a standard concentration-fluorescence response curve on the HPLC.

To order or request a quote for either of these standards or the UHPLC column please e-mail info@ludger.com