The Duolink II reagent kit has a new and improved formula and in combination with fewer steps and a significantly shorter protocol it becomes more user-friendly and efficient. Experi-ment and analysis can be completed within the same day.

The Duolink ImageTool is a dedicated and easy to use imaging software tool optimized for the analysis of cells and tissue sections processed with the Duolink reagents. Each signal, generated from the reagents, can objectively and accurately be quantified by the software, enabling single cell statistical analysis of expression levels in tissue or cell populations. The results can easily be exported to Excel for further evaluation and result images can be ex-ported to TIFF for use in publications.

The Duolink II Probemaker kit enables quick and convenient conjugation of the PLA® oli-gonucleotide arms directly to primary antibodies, eliminating the need for PLA probes based on secondary antibodies. This feature allows the use of same species monoclonal or poly-clonal antibodies in a Duolink assay and also opens up the possibility to study homodimeric protein complexes.
The Duolink product line is based on Olink Bioscience proprietary in situ PLA, a proximity ligation assay technology that extends the capabilities of traditional protein techniques to include direct detection of proteins, protein-protein interactions and post-translational modifi-cations with unparalleled specificity and sensitivity. Target proteins are detected at the sin-gle molecular level, allowing digital quantification and information about the exact localiza-tion of the targets. Furthermore, the proteins can be studied at physiological expression lev-els in fixed cells and tissue sections.