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news.Heidelberg German Cancer Research Center (DKFZ) conducted a study using Roche's xCELLigence MP Instrument to quantify human kinome RNAi for assaying the effects of small interfering RNA (siRNA) transfections with electrical impedance as output.
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The instrument makes use of a software and E-Plates 96 to measure electronic cell impedance using sensor electrodes.
Continual detection of changes in cell behavior using 96-well culture plates is enabled by computer-controlled signal generation and automatic frequency scanning.
The impedance measures cell number, quality of the cells’ interaction with their substrate and aids to assess cell status, including cell morphology, cell adhesion and cell viability.
The study showed that xCELLigence System measurements correlate with results obtained using endpoint analysis of Promega’s CellTiter-Blue and Roche’s WST-1 Cell Proliferation Reagent for assaying cell viability, as well as qRT-PCR for quantifying gene expression.
The data obtained with the xCELLigence System allowed to identify the timing after transfection where the effect was maximal and to sort genes according to these time points.