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news.ForteBio, a division of Pall Life Sciences, has launched Dip and Read Ni-NTA biosensor for use on its Octet and BLItz instrumentation platforms.
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The new biosensor is pre-immobilized with novel nickel-charged tri-nitrilotriacetic (Tri-NTA) groups enabling direct method for quantitation and kinetic analysis of polyhistidine-tagged (HIS-tagged) biomolecules.
ForteBio division marketing vice president Christopher Silva said nickel provides an excellent binding medium for the stable capture of HIS-tagged proteins as part of quantitation and characterization studies.
"However, standard formats for using this medium have been cumbersome, time-consuming and expensive. Now, our new Ni-NTA biosensor enables scientists to quickly and easily analyze HIS-fusion proteins with unsurpassed ease of use and workflow efficiency," Silva added.
The Ni-NTA biosensor, designed for use in buffer and diluted complex media, can also be regenerated up to 10 times via a standard low-pH protocol in as little as two minutes for various applications such as acquisition of replicate data and bucket-based screening applications.
ForteBio’s Octet family of products is based on the company’s BioLayer Interferometry technology, which incorporates disposable optical Dip and Read biosensors that measure multiple interactions in parallel, without the use of detection agents.