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news.DiscoveRx has developed a rapid, quantitative fluorescence detection assay for PI3 Kinase suitable for HTS applications.
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The advantage of this assay over other formats is that it generates a positive readout in direct proportion to ADP accumulated. This is particularly useful in determining kinase and inhibitor kinetics such as substrate Km and inhibitor Ki.
Anna Christensen, product manager for Kinases at DiscoveRx, said: “The ADP Hunter HS based PI3K assay has been shown to give the highest degree of sensitivity and simplicity in the market.”