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news.AnaSpec, the North American division of Eurogentec, has introduced anti-5-methylcytidine (Clone 33D3), the monoclonal antibody for use in epigenetics research. Purified from ascites, the isotype antibody is supplied as a 1mg/ml solution (in PBS) and is available in three sizes (0.1mg, 0.5mg and 1mg).
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AnaSpec said that the IgG1 / ? isotype 5-Methylcytidine is a modified base found in the DNA of plants and vertebrate. DNA methylation is a post-replication process involved in the establishment of genomic imprinting, in the control of gene expression and of differentiation. Alterations of DNA methylation pattern are associated with carcinogenesis.
A global DNA hypomethylation is often detected in tumor tissues, associated with local hypermethylation sites. Since this antibody discriminates between the modified base, 5-MeCyd and its normal counterpart, cytosine; it is a useful tool for gene promoter methylation analysis.
Anti-5-Methylcyidine from Clone 33D3 can be used in applications such as Methylated DNA Immunoprecipitation (MeDIP), MeDIP-on-chip assay, immunofluorescence, flow cytometry and immunohistochemistry. Histopathological analysis of tissue samples can be performed without having to destroy cells through DNA extraction procedures.
AnaSpec has said that this antibody has been used to detect alterations in the urinary excretion of nucleosides from cancer patients, to visualize the distribution of methyl-rich regions along human chromosomes, to quantify in situ differences between normal and malignant cells from peripheral blood as well as on tissue sections.