University of Navarra Deploys Wyatt Technology Dynamic Light Scattering

Wyatt Technology said that DynaPro facilitated the detection of aggregation in early stages of micellisation of pluronic polymeric surfactant due to temperature effects, pH, salinity concentration and composition.

DynaPro offers scientists the ability to measure small volumes of dilute protein samples. Dynamic Light Scattering (DLS) instruments are tools for scientists examining protein structure.

Wyatt Technology said that DLS technology detects aggregation early due to temperature effects, pH, salinity concentration or composition. The temperature control capability of the DynaPro allows for accelerated stability, thermal melting and temperature studies of multiple samples to be performed in parallel and with improved response time.

For the application at the University of Navarra, the system was used for the micellisation of a Pluronic polymeric surfactant with temperature.

The results demonstrate that, at a low temperature, the polymeric surfactant exists in monomer form. The onset of micellisation can be easily perceived both in the intensity and in the hydrodynamic radius obtained by the cumulants method of Wyatt Technology’s Dynamics software. The regularisation analysis of Dynamics provides the size spectrum of the sample and reveals a new peak corresponding to the micelles.