The technique applied was Hydrophobic Interaction Chromatography (HIC), which seems to work with antibody-drug-conjugates (ADC’s) with great success, opening up new capabilities in the field of effective cancer treatment. The best separation conditions were found fast with the newest DryLab4-HPLC-method development software, developed by Molnár-Institute, Berlin. Analysis time was typically less than 10 min, method development time is approximately half a day. Read more in our literature section!