The MOLNÁR-INSTITUTE for Applied Chromatography is proud to announce its participation as a Bronze Sponsor, scientific contributor, and exhibitor at the upcoming International Symposium on High Performance Liquid Phase Separations (HPLC 2025). Taking place in Bruges, Belgium, HPLC 2025 will feature cutting-edge research presentations, technical workshops, and exhibitions from global leaders in the industry.

The DryLab® MOLNÁR-INSTITUTE stand at Booth 14 in the Exhibition Zone of the BMCC Bruges Conference Center will present all the new developments – updated modules and features of the DryLab®4 separation modelling software. For over 40 years, DryLab® has supported method development in modern high-performance liquid chromatography (HPLC), contributing to improved efficiency and reliability in life science applications across pharmaceutical, food, and environmental analysis.

With its strong focus on innovation in cutting-edge industry solutions, laboratory automation, and data-driven analytical techniques, HPLC 2025 provides an essential platform for advancing the science of separation and enhancing laboratory performance – areas where The MOLNÁR-INSTITUTE continues to lead and innovate.

Learn how to work smarter in chromatography! Visit the MOLNÁR-INSTITUTE booth for live demonstrations, join cutting-edge presentations, and meet the team behind one of the industry’s leading method development platforms.

Stationary Phase Selection in MS-analysis of Oxysterols

As part of its commitment to advancing analytical technology, the DryLab® scientific team will present two contributions highlighting model-assisted applications in chromatographic method development.

The first study, led by Andrea Castellaneta from Aldo Moro University of Bari, Italy, is titled “Tackling the Challenge of Oxysterols Separation by High-Performance Liquid Chromatography: The Application of Analytical Design Space Modelling to Explore the Capabilities of Different Stationary Phases.” This work was conducted in collaboration with Dr Imre Molnár, president, and Dr Hans-Jürgen Rieger, vice-president of the MOLNÁR-INSTITUTE.

The study demonstrates the successful use of separation modelling to enhance the robustness of hyphenated LC-MS methods for the analysis of oxysterol compounds. Using DryLab® software, the chromatographic separation was optimised across C18 and cyanopropyl stationary phases, resulting in a robust method without the need for derivatisation agents – a significant improvement over previous approaches. Overall, these results have contributed to the development of a more reliable RPLC-FTMS method, offering a robust and efficient platform with minimal sample preparation requirements.

Tackling pH challenges in RPC

The second scientific contribution to the symposium will be a poster summarising a recent study published in the Journal of Chromatography A, titled “Extended Multidimensional Design Space Studies of Volatile and Non-Volatile Buffer Systems.” The work was led by Arnold Zöldhegyi, Senior Scientist at the Molnár-Institute, in collaboration with Dr Imre Molnár and other prominent researchers, Dr Krisztián Horváht from the University of Pannonia and Dr Róbert Kormány from Egis Pharmaceuticals Plc. in Hungary.

The study addresses the challenges of chromatographic selectivity and the choice of pH modifiers in the separation of ionizable compounds by HPLC. Using DryLab®4 modelling within an Analytical Quality by Design (AQbD) framework, the team developed and compared three-dimensional (tG-T-pH) separation models for terazosin and selected impurities across a pH range established using both volatile and non-volatile buffers.

The results revealed not only expected equivalences within overlapping Method Operable Design Regions (MODRs), but also highlighted buffer-specific selectivity differences with significant implications for method robustness. This study underscores the critical role of buffer selection in developing reliable, high-performance separation methods.

Don’t miss the chance to connect, learn, and be inspired — see you in Bruges.