The first part of the assay is a non lytic fluorescence assay (CellTiter-Fluor™ Cell Viability Assay) that measures the relative number of the live cells in a culture population after experimental manipulation. The CellTiter-Fluor Assay measures a conserved and constitutive protease activity within live cells and therefore serves as a marker of cell viability. The substrate enters intact cells where it is cleaved by the live-cell protease to generate a fluorescent signal proportional to the number of living cells. The second part of the assay uses the ONE-Glo Lucifease Assay System to quantify firefly luciferase reporter gene expression from cells made to express this reporter enzyme.

Ideally suited for high- and ultra high-throughput applications, the volumes of each assay component can be scaled to meet throughput needs and is amenable to automation up to 1,536-well format. The ONE-Glo + Tox Assay contains a new fluoroluciferin substrate, resulting in a reagent that is more stable and tolerant to sample components. Luminescence can be measured with a microplate reader or CCD imager.

For more information please visit: www.promega.com/oneglotox