ELISA, Multiplex, and Flow Cytometry Testing

CTL’s enzyme-linked immunospot (ELISPOT) assay expertise is complemented by its ELISA testing, multiplex cytometric bead array (CBA) and fluorescence-activated cell sorting (FACS) services.

ELISA testing can be used to extract additional information on the production of antibodies or other cytokines from the same sample without requiring the use of additional blood draws. Similarly, flow cytometry can be used to determine what cell population is prevalent in the monitored response.

It is also an invaluable tool when T-cell responses are excessively large such as when they are too numerous to count using the ELISPOT method. All of these assays can be used in conjunction with the ELISPOT assay.

The supernatant can be collected from ELISPOT plates and used in other test systems such as ELISA assays and CBAs. Together, these technologies can provide a more comprehensive view of the subject’s immune response.

CTL also offers standardised ELISA protocols for multiple species. In addition, CTL’s good laboratory practice (GLP) laboratory has diversified its service portfolio to include CBAs, intracellular cytokine staining, surface marker staining via flow cytometry, and other assay types.