Detection of antigen-specific CD8 cells relies on the use of peptides that can bind to human leukocyte antigen (HLA) Class I molecules.

There is extensive knowledge on individual HLA-alleles’ peptide binding requirements and for many antigens, immunogenic peptides have been defined.

The 32 individual peptides that comprise the CEF peptide pool represent such well-defined peptide determinants for cytomegalo, epstein barr, and flu virus. CTL tested 42 healthy human donors on the accuracy of these peptide predictions.

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