Tosoh Bioscience, a provider of chromatographic solutions for the separation of biomolecules, announced today the introduction of TSKgel UP-SW30000-LS size exclusion chromatography (SEC) columns. These columns are the latest addition to the TSKgel UP-SW series and are now available for sale in the Europe, Middle East and Africa (EMEA) region from Tosoh Bioscience.

TSKgel UP-SW3000-LS columns are packed with 2μm silica-based particles and have a pore size of 25nm. They resemble the TSKgel UP-SW3000 columns, which are well-established for biotherapeutics analyses. The key feature of the TSKgel UP-SW3000-LS columns is their outstanding low noise levels when used with advanced detection methods such as multi-angle light scattering (MALS) and mass spectrometry (MS). When these columns are coupled with MALS, the low noise levels yield high signal-to-noise ratios and improve the sensitivity of detection. For mass spectrometry applications, the columns will increase electrospray ionisation efficiency and enhance overall MS performance and instrument uptime.

TSKgel UP-SW3000-LS columns are available in 4.6mm ID with 15cm or 30cm length. The 15cm column offers a shortened analysis time, while the 30cm column delivers dramatically increased peak parameters such as efficiency, asymmetry and resolution between monoclonal antibodies’ fragments, monomers and aggregates. In addition, the TSKgel UP-SW3000-LS columns demonstrate high reproducibility from injection-to injection and across lot-to-lot and column-to-column. These UHPLC columns can also be operated using optimised HPLC systems, which allows for a smooth method transfer from high-performance liquid chromatography (HPLC) to ultra-high-performance liquid chromatography (UHPLC).

Tosoh Bioscience is very excited about the introduction of the TSKgel UP-SW3000-LS columns. For many years, analytical scientists have come to rely on TSKgel SEC columns for their biomolecular analysis. This next generation of columns offers all the first-in-class qualities that users have come to expect from TSKgel columns for protein characterisation and quality control. Complementation with MALS or MS detection offers highly sensitive mass analysis of sample components, which is increasingly employed to identify impurities in biotherapeutics. The TSKgel UP-SW3000-LS columns were developed to enable the effortless combination of SEC with MALS or MS while maintaining high separation performance.