Trinean - Pharmaceutical Business review

Pharmaceutical Business review is using cookies

Continue Learn More

Trinean

UV-VIS Spectral Analysis of DNA/RNA, Protein or Small Compounds

WHITE PAPERS

1.10 cDrop Protocol for TRIzol RNA Extract Quantification and Content QC

1.14 Droplet UV/VIS Quantification of Native Proteins in Biopharmaceutical Processing

1.17 Maxwell®16 Blood purification Kit App and Maxwell®16 LEV Blood DNA Kit App

1.19 Qiagen® EZ1 Tissue DNA app

View all Whitepapers

PRODUCTS & SERVICES

DropPlate 96

DropSense 96

DropSense96™

Xpose™

View all Products & Services

LATEST PRESS RELEASES

Trinean

Trinean develops and produces a new approach for high throughput full scan UV-VIS spectral analysis of microliter droplets of DNA/RNA, protein or small compounds.

Trinean is a premium instrumentation company, pushing the boundaries of micro-volume molecular spectroscopy to a next level, with a strong focus on accuracy, throughput and sample quality assessment. Trinean brings value to its customers by combining first-in-class analytical software with proprietary, standardized read-out platforms thereby helping scientists around the globe to save time and money on costly samples and reagents while gaining in accuracy and speed.

Trinean

Founded in 2006 as a spin-out of the Ghent University, Belgium and Imec Leuven, Belgium, the company develops and commercializes analytical platforms for precise quantification of biological samples and in-depth sample purity analysis. Trinean’s UV/VIS readers combined with microfluidic sample carriers, allow for fast UV/VIS analysis of droplet samples (2µl), extracting accurate quantitative information while detecting sample contaminants. The Trinean technology is the method of choice for QC and QA of DNA, RNA or Protein samples, given its sample purity analysis, dynamic range and operational efficiency.

Trinean products include two platforms: the Xpose™ for ‘Touch & Go’ bio-molecule quantification and the DropSense96™, analyzing up to 96 samples, using microfluidic chips for standardized sample analysis (DropPlates and Xpose Slides). The Trinean platform is complemented with a software toolbox (including DropControl, DropQuant, cDrop™) for improved data interpretation and lab-automation.

More information at www.trinean.com

trinean

DropPlate 96

The TRINEAN DropPlate 96 has been designed to be read with the DropSense 96.

This unique design resolves the following issues when measuring small droplets of liquids:No evaporation during dispensing and measuringSmall path lengths allowing measurement of small volumes (1,5 - 2,5 µL)Path length for high concentrations: 0,2mmPath length for low concentrations: 1mmWide dynamic range: 2ng to 3500ng dsDNANo meniscus as with microplatesDisposable microfluidic chip with sample 96 positions.Input…

MORE ABOUT THIS PRODUCT

BROCHURE AVAILABLE

DropSense 96

The TRINEAN technology allows UV/VIS spectroscopy in droplets of samples (0,5 to 2,5 µL) and is compatible with standard micoplate liquid handlers and robots.

The technology is a combination of the DropSense 96 polychromatic microplate reader with the DropPlate 96 consumable. This technology allows quantification of DNA/RNA and proteins in small samples, without utilisation of a manual reader, or diluting of the sample in a UV 96 microplate.The systems provide a full spectrum scan of each sample and generate…

MORE ABOUT THIS PRODUCT

BROCHURE AVAILABLE

DropSense96™

The DropSense™ 96 platform offers dye-free quantification of DNA, RNA or protein samples in a micro-volume 96well format. Precise UV/VIS spectral droplet (2µl) analysis combined with unique cDrop™ spectral content profiling software allows specific quantification and detection of contaminants as in-depth content QC. Additional software tools are available for robot integration and compliance in regulatory environments.

The Trinean Dropsense™96 micro-volume reader is designed for high throughput quantification of native nucleic acid or protein samples. Combined with its unique microfluidic 96-well DropPlates, micro-volume (2µl) quantification is made possible in a fully automated workflow. A complete software toolbox is available for detailed sample QC, robot integration and compliance in regulatory environments.Key featuresDroplet samples:…

MORE ABOUT THIS PRODUCT

BROCHURE AVAILABLE

Xpose™

The Xpose™ 'Touch & Go' reader is a next generation micro-volume QC tool offering high-speed DNA, RNA and proteins quantification and in-depth contamination analysis.

Simply load your samples (2µl) onto the Xpose slides on your bench, insert the slide into the Xpose reader and select the quantification app on the touch screen. No repetitive drop & clean actions. Pre-defined apps on the touch screen allow selection of traditional spectroscopy (A260/A280) or advanced spectral content profiling able to quantify isolated…

MORE ABOUT THIS PRODUCT

BROCHURE AVAILABLE

1.5 Specific dsDNA Quantification UV/VIS-based cDrop™ Method vs Picogreen® Fluorescent Assay

Precise quantification and normalization of isolated genomic DNA is critical for advanced molecular testing in life science research and molecular diagnostics. UV-Vis based quantification has long been the method of choice, mainly because of its convenience. However, determining the DNA concentration based on the absorbance at 260nm suffers from interference of components such as RNA, proteins and phenols. Alternative methods, such as the PicoGreen® based Quant-iT™ assay, based on fluorescence enhancement upon binding with dsDNA have been developed to address this issue.

DOWNLOAD WHITEPAPER

1.3 Micro-volume Protein Quantification using the DropSense96 Droplet Plate Reader

The measurement of protein concentration in aqueous samples is an important assay in biochemistry research and protein production facilities. Spectrophotometric protein quantification assays are commonly used methods to rapidly determine the concentration of a protein. They utilize the direct UV absorbance of the protein at 280nm in combination with its extinction coefficient or an indirect dye-based methods like BCA, Lowry and Bradford assays.

DOWNLOAD WHITEPAPER

1.2 DropSense96 and its DropPlates, a Versatile Tool for Nucleic Acid and Protein Droplet Quantification

As the demand for molecular tests increases, automated systems for biomolecule isolation and sample analysis are becoming popular. These facilitate integration of the complete molecular test in a full automatic set-up with liquid robots and bar-coded sample tracking resulting in a significant higher throughput while minimizing tedious manual repetitive tasks. The Trinean technology is a unique combination of the DropSense96 droplet plate reader, the 96well DropPlate microfuidic consumables and the DropQuant analytical software enabling direct quantification of small biomolecule samples in these high throughput, automated workflow.

DOWNLOAD WHITEPAPER

1.14 Droplet UV/VIS Quantification of Native Proteins in Biopharmaceutical Processing

Biopharmaceuticals are mainly produced by microbial fermentation or in animal cell cultures. As a consequence, a cascade of procedures including purification and characterization are required throughout the processing chain to obtain the protein at the desired level of purity and potency. Within these processes, quantification of the protein of interest is mandatory at critical steps to maintain confidence in the processing results and fidelity in the end product.

DOWNLOAD WHITEPAPER

1.20 Quantification of His-tagged IgG antibodies after IMAC Ni-column purification

Trinean introduces a new cDrop™ application suited for the quantification (OD280) of IgG antibodies and derivatives like Fab fragments during purification by IMAC Ni-columns using imidazole-containing buffers.

DOWNLOAD WHITEPAPER

Dye-free quantification of Oragene®/saliva DNA extracts using the Trinean DropSense96® Droplet Reader and cDrop™ Spectral Analysis Software

Saliva samples collected with Oragene® self-collection kits from DNA Genotek offer a non-invasive and proven method for accessing high quality and quantity DNA for genomic testing and analysis. Proper quantification of the extracted DNA is a critical step for most downstream applications. Trinean has developed a specific quantification protocol for DNA from Oragene/saliva samples using the DropSense96® droplet reader and the cDrop™ spectral decomposition software. A comparative analysis using a PicoGreen® fluorescent quantification assay shows a comparable specificity for dsDNA quantification. Since the read-out of 96 samples with the DropSense96 is performed in 5 minutes without any prior mixing or incubation, this technology is a viable alternative to fluorescent quantification assays.

DOWNLOAD WHITEPAPER

1.17 Maxwell®16 Blood purification Kit App and Maxwell®16 LEV Blood DNA Kit App

A spectral content profiling application on the cDrop™ software is available for quantification and contamination analysis of DNA isolations from blood using the Maxwell® 16 Blood DNA purification Kit and The Maxwell®16 LEV Blood DNA Kit. In this note we describe how to use this application and how to export and interpret the results.

DOWNLOAD WHITEPAPER

1.7 Quantifying Amplicons in Non-Purified PCR Reactions: Direct Assessment of Product Yield

The polymerase chain reaction (PCR) is a fundamental tool in molecular biology and clinical testing. Most genetic tests rely upon the power of PCR to enrich specific target-sequences prior to analysis. Analytical methods like sequencing are relatively expensive and labs want to minimize the resources spent on samples that don't meet the requirements of concentration and quality to deliver a reliable result.

DOWNLOAD WHITEPAPER

1.19 Qiagen® EZ1 Tissue DNA app

A spectral content profiling application on the cDrop™ software is available for quantification and contamination analysis of DNA isolations from cells using the Qiagen® EZ1 Tissue DNA kit. In this note we describe how to use this application and how to export and interpret the results.

DOWNLOAD WHITEPAPER

1.10 cDrop Protocol for TRIzol RNA Extract Quantification and Content QC

This cDrop protocol is developed for specific RNA quantification of extracts from a variety of sample origins (human, animal, plant) using extraction kits based on the one-step extraction method using a guanidinium thiocyanate-phenol- chloroform reagent-mix developed by Chomczynski (1). Commercial available variants include the TRIzol® kit (Invitrogen), TRI Reagent® (MRC), RNAzolTM (MRC) and RNA STAT-60TM (Tel-Test).

DOWNLOAD WHITEPAPER

All-Purpose Protein Sample QC Using a Novel Micro-Volume Platform with Spectral Content Profiling Software

Accurate total protein quantitation of purified proteins, cell lysates, affinity-column fractions or other complex protein samples is crucial for robust biochemical testing during biopharmaceutical development as well as production. As the presence of chemicals doesn't allow simple A280 protein quantification, more labor-intensive alternatives for quantification are used. Examples are the colorimetric Bradford or BCA assay, which negate to a large extent the presence of contaminants but require multiple steps and show variation depending on the sample composition and time point of measuring within the ongoing reaction.

DOWNLOAD WHITEPAPER

Precise Nucleic Acid Quantification Using Lab-on-a-chip Spectroscopy and the cDrop Quantification Algorithm

Precise quantification and quality control of isolated genomic DNA is critical for the success of downstream applications such as next-generation sequencing. Traditionally, two quantification methods have been used routinely: (1) UV/Vis spectroscopy and (2) fluorescence-based assays (i.e. PicoGreen) that utilize DNA intercalating agents.

DOWNLOAD WHITEPAPER

Sample Processing for the UK Biobank Genotyping Project

A project was initiated in 2013 to perform SNP genotyping on all UK Biobank participants. The aim of the project (which was to be completed within an 18-24 month timeframe) was to generate high quality genotype data and to make these data available for researchers, via the UK Biobank Showcase system. This poster describes the sample processing workflow, presents a comparison between the two quantification methods used in the project (Trinean DropSense® 96 and PicoGreen) and provides a summary of the genotyping metrics for the (approx.) first 180,000 samples analysed.

DOWNLOAD WHITEPAPER

Droplet UV/VIS Quantification of Native Proteins in Biopharmaceutical Processing

Biopharmaceuticals are mainly produced by microbial fermentation or in animal cell cultures. As a consequence, a cascade of procedures including purification and characterization are required throughout the processing chain to obtain the protein at the desired level of purity and potency. Within these processes, quantification of the protein of interest is mandatory at critical steps to maintain confidence in the processing results and fidelity in the end product.

DOWNLOAD WHITEPAPER

Contact Trinean

First Name*

Last Name*

Email*

Telephone Number

Role In Purchasing*

Job Title*

Job Function*

Company*

Company Activity*

Country*

Comments

Optional Data

Almost there, complete this form and hit the submit button to complete your registration.

Address Line 1

Address Line 2

City

County/State

Postcode/ZIP Code

Fax

Company Turnover

No of Employees

Email Updates

Please confirm which of the FREE email services you would like to receive from us, you can unsubscribe at any time:

Pharmaceutical Business review Daily Update

Pharmaceutical Business review Weekly Round-up

magazinePharmaceutical Business review Magazine

Confirmation

Finally, complete a quick security check and confirm terms and conditions:

I have read and accept the terms and conditions and privacy policy.

We'd like to keep you informed about relevant promotions, products and services, if you would like not to receive these, check this box.

If you would like to hear from carefully selected third party companies, check this box

Pharmaceutical Business review is using cookies

Research & Development

Patient Enrollment

Drug Filing

Manufacturing

Packaging

No data found

Trinean

UV-VIS Spectral Analysis of DNA/RNA, Protein or Small Compounds

Trinean

Top Products from Trinean

The TRINEAN DropPlate 96 has been designed to be read with the DropSense 96.

The TRINEAN technology allows UV/VIS spectroscopy in droplets of samples (0,5 to 2,5 µL) and is compatible with standard micoplate liquid handlers and robots.

The Xpose™ 'Touch & Go' reader is a next generation micro-volume QC tool offering high-speed DNA, RNA and proteins quantification and in-depth contamination analysis.

1.5 Specific dsDNA Quantification UV/VIS-based cDrop™ Method vs Picogreen® Fluorescent Assay

1.3 Micro-volume Protein Quantification using the DropSense96 Droplet Plate Reader

1.2 DropSense96 and its DropPlates, a Versatile Tool for Nucleic Acid and Protein Droplet Quantification

1.14 Droplet UV/VIS Quantification of Native Proteins in Biopharmaceutical Processing

1.20 Quantification of His-tagged IgG antibodies after IMAC Ni-column purification

Trinean introduces a new cDrop™ application suited for the quantification (OD280) of IgG antibodies and derivatives like Fab fragments during purification by IMAC Ni-columns using imidazole-containing buffers.

Dye-free quantification of Oragene®/saliva DNA extracts using the Trinean DropSense96® Droplet Reader and cDrop™ Spectral Analysis Software

1.17 Maxwell®16 Blood purification Kit App and Maxwell®16 LEV Blood DNA Kit App

1.7 Quantifying Amplicons in Non-Purified PCR Reactions: Direct Assessment of Product Yield

1.19 Qiagen® EZ1 Tissue DNA app

A spectral content profiling application on the cDrop™ software is available for quantification and contamination analysis of DNA isolations from cells using the Qiagen® EZ1 Tissue DNA kit. In this note we describe how to use this application and how to export and interpret the results.

1.10 cDrop Protocol for TRIzol RNA Extract Quantification and Content QC

All-Purpose Protein Sample QC Using a Novel Micro-Volume Platform with Spectral Content Profiling Software

Precise Nucleic Acid Quantification Using Lab-on-a-chip Spectroscopy and the cDrop Quantification Algorithm

Sample Processing for the UK Biobank Genotyping Project

Droplet UV/VIS Quantification of Native Proteins in Biopharmaceutical Processing

Contact Trinean

Optional Data

Email Updates

Confirmation

Trinean

UV-VIS Spectral Analysis of DNA/RNA, Protein or Small Compounds